When you analyzed the results of your gel, did any of your experimental samples contain dyes that did not match the four reference dyes? For example, did any of your samples produce?
Look at the structures of the dyes pictured here. Which of these dyes would migrate similarly to the dyes you examined in this lab? Why?
All of them because they are all the same color that we tested. They won't move at the same speed, but they should be similar.
Many popular dry dog foods and dog treats have FD&C dyes among their ingredients. For example, Beneful dry food contains Yellow 5, Red 40, Yellow 6, and Blue 2, and Snausages Breakfast Bites contain Red 40 lake, Yellow 6 lake, and Yellow 5 lake. (Lake dyes are the insoluble forms of the FD&C dyes.)
Why do dog food manufacturers put artificial food colors in dog food?
Are there any reasons why artificial food colors might be preferable to natural food colors?
Some natural food colors are not appetizing. There are also some natural food colorings made out of bugs, and that is very unappetizing.
What two factors control the distance the colored dye solutions migrate?
The length/size one the DNA, and how well the DNA was injected into the gel.
What force helps move the dyes through the gel?
Electricity
What component of the electrophoresis system causes the molecules to separate by size? Explain
The gel is the key to this working. The gel has little holes in it, and these holes are just large enough for DNA to move through them. The small DNA has no problem getting through the gel, but the large DNA has a lot more trouble. This is why the large DNA moves a lot slower than the small DNA.
Agarose electrophoresis is commonly used to separate molecules of DNA. Explain how you expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate.
Well clearly the 5000 dalton DNA is going to move very slowly, while the 500 dalton DNA will rapidly move to the other end of the gel.
Some natural food colors are not appetizing. There are also some natural food colorings made out of bugs, and that is very unappetizing.
What two factors control the distance the colored dye solutions migrate?
The length/size one the DNA, and how well the DNA was injected into the gel.
What force helps move the dyes through the gel?
Electricity
What component of the electrophoresis system causes the molecules to separate by size? Explain
The gel is the key to this working. The gel has little holes in it, and these holes are just large enough for DNA to move through them. The small DNA has no problem getting through the gel, but the large DNA has a lot more trouble. This is why the large DNA moves a lot slower than the small DNA.
Agarose electrophoresis is commonly used to separate molecules of DNA. Explain how you expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate.
Well clearly the 5000 dalton DNA is going to move very slowly, while the 500 dalton DNA will rapidly move to the other end of the gel.
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