pGLO Lab Conclusion
Plate
|
Number of colonies
|
Color of colonies under room light
|
Color of colonies under room light
|
-pGlO LB
|
0 (lawn)
|
grey
|
grey
|
-pGLO LB/amp
|
0 (lawn)
|
grey
|
grey
|
+pGLO LB/amp
|
75
|
tan
|
tan
|
+pGLO LB/amp/ara
|
250
|
tan
|
green
|
2.
|
What two new traits do your transformed bacteria have?
| |
-Ability to be resistant to Amposilin
-Ability to glow
| ||
3.
|
Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
| |
-25 mL in our test plate=250 germ colonies
| ||
-100 mL in our test plate= ? germ colonies
| ||
THE ANSWER IS 1000 (250x4)
| ||
4.
|
What is the role of arabinose in the plates?
| |
It allowed the RNA to transfer into the germs, causing them to glow.
| ||
5
|
List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
| |
-Testing a protein in jellyfish that makes jellyfish.
- Using green fluorescent proteins to tag the expression of genes
-Making Cats glow
| ||
6.
|
Give an example of another application of genetic engineering.
-Mass production of insulin, follistim, antibodies, and vaccines
| |
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